A CLINICIAN´S GUIDE TO SPERM DNA AND CHROMATIN DAMAGE

1. Sperm Chromatin: An Overview
2. Sperm Nucleoproteins (Histones and Protamines)
3. Sperm Nuclear Architecture
4. Sperm Chromatin Structure Assay (SCSA®): Evolution from Origin to Clinical Utility
5. TUNEL Assay
6. TUNEL Assay by Benchtop Flow Cytometer in Clinical Laboratories
7. The Comet Assay
8. Sperm Chromatin Dispersion (SCD) Assay
9. Cytochemical Tests of Sperm Chromatin Maturity
10. Is There an Optimal Sperm DNA Test?
11. Oxidative Stress
12. Apoptosis in Ejaculated Spermatozoa and in the Normal and Pathological Testes: Abortive Apoptosis and Sperm Chromatin Damage
13. Defective DNA Repair in Spermiogenesis
14. Defective Spermatogenesis and Sperm DNA Damage
15. Sperm Chromatin and Lifestyle Factors
16. Cancer and Sperm DNA Damage
17. Sperm Chromatin and Environmental Factors
18. Sperm DNA Damage and Oocyte Repair Capability
19. Experimental Studies on Sperm DNA Fragmentation and Reproductive Outcomes
20. Sperm DNA and Natural Pregnancy
21. Sperm DNA and ART (IUI, IVF, ICSI) Pregnancy
22. Sperm DNA and Pregnancy Loss After IVF and ICSI
23. Sperm DNA Tests Are Clinically Useful: Pro
24. Sperm DNA Tests Are Clinically Useful: CON
25. Antioxidant Therapy
26. Varicocelectomy
27. Physiological Intracytoplasmic Sperm Insemination Based on Hyaluronic Acid-Binding Ability
28. Advanced Sperm Processing/Selection Techniques
29. Use of Testicular Sperm for ICSI: Pro
30. Debate on the Use of Testicular Sperm for ICSI: Con
31. Strategies to Diminish DNA Damage in Sperm Samples Used for ART
32. Sperm DNA Testing: Where Do We Go from Here?

This comprehensive, up-to-date text, which brings together the key practical elements of the rapidly evolving field of sperm DNA and chromatin abnormalities, is divided thematically into five main sections. Part I discusses human sperm chromatin structure and nuclear architecture, while part II presents laboratory evaluation of sperm DNA damage, including SCSA, SCD, TUNEL and Comet assays, and cytochemical tests. Biological and clinical factors in the etiology of sperm DNA damage are discussed in part III, including oxidative stress, abortive apoptosis, cancer, and environmental and lifestyle factors. Part IV presents clinical studies on the utility of sperm DNA damage tests, both with natural and ART-assisted pregnancies, and debates the clinical utility of such tests. Finally, part V discusses current treatment options, such as antioxidant therapy, varicocelectomy, advanced sperm processing techniques and the use of testicular sperm.
We are now beginning to better understand the unique organization of the sperm chromatin, as well as the nature and etiology of sperm DNA damage. Written and edited by worldwide experts in andrology, A Clinician's Guide to Sperm DNA and Chromatin Damage is an excellent resource for reproductive medicine and REI specialists, urologists, reproductive biologists and any professional working with the infertile male.

Features
• A comprehensive, up-to date guide to sperm DNA and chromatic damage for the clinician treating the infertile maleSections cover sperm chromatin structure, lab evaluation of chromatin and DNA damage, biological and clinical factors, clinical studies on the utility of sperm DNA damage testing, and current treatment options
• An excellent resource for reproductive medicine and REI specialists, urologists and any professional working with the infertile male

Authors
• Armand Zini, MD, FRCSC, Professor, Department of Surgery, Division of Urology, St. Mary’s Hospital Center, Montreal, Québec, Canada
• Ashok Agarwal, PhD, HCLD, Professor, Lerner College of Medicine of Case Western Reserve University, Director, Andrology Center, Director, American Center for Reproductive Medicine, Department of Urology, Cleveland Clinic Foundation, Cleveland, Ohio, USA