ANTIGEN PROCESSING. METHODS AND PROTOCOLS. 2ND EDITION

ANTIGEN PROCESSING. METHODS AND PROTOCOLS. 2ND EDITION

Editorial:
SPRINGER
Año de edición:
Materia
Genética
ISBN:
978-1-4939-9449-6
Páginas:
458
N. de edición:
2
Idioma:
Inglés
Ilustraciones:
79
Disponibilidad:
Disponible en 2-3 semanas

Descuento:

-5%

Antes:

176,79 €

Despues:

167,95 €

1. Purification of Large Cytosolic Proteases for In Vitro Assays: 20S and 26S Proteasomes
2. Analysis of Proteasome-Generated Antigenic Peptides by Mass Spectrometry
3. Trimming of MHC Class I Ligands by ERAP Aminopeptidases
4. Peptide Trimming for MHC Class I Presentation by Endoplasmic Reticulum Aminopeptidases
5. Testing the Impact of Protease Inhibitors in Antigen Presentation Assays
6. In Vitro Studies of MHC Class I Peptide Loading and Exchange
7. Measuring Synthesis and Degradation of MHC Class I Molecules
8. Biochemical Analysis of Naturally Processed Antigenic Peptides Presented by MHC Class I Molecules
9. A SIINFEKL-Based System to Measure MHC Class I Antigen Presentation Efficiency and Kinetics
10. Purification and Identification of Naturally Presented MHC Class I and II Ligands
11. Identification of MHC Ligands and Establishing MHC Class I Peptide Motifs
12. Quantitating MHC Class I Ligand Production and Presentation Using TCR-Like Antibodies
13. Identifying Antigens Recognized by Cytolytic T Lymphocytes on Tumors
14. A Flow Cytometry-Based Approach to Unravel Viral Interference with the MHC Class I Antigen Processing and Presentation Pathway
15. Evaluating CD8+ T Cell Responses In Vitro
16. Recombinant Poxviruses: Versatile Tools for Immunological Assays
17. Monitoring Intracellular Routing of Internalized Antigens by Immunofluorescence Microscopy
18. Monitoring MHC Ubiquitination by MARCH Ubiquitin Ligases
19. Monitoring MHC-II Endocytosis and Recycling Using Cell-Surface Protein Biotinylation-Based Assays
20. Monitoring Protein Endocytosis and Recycling Using FACS-Based Assays
21. In Vitro Digestion with Proteases Producing MHC Class II Ligands
22. Assembly, Intracellular Transport, and Release of MHC Class II Peptide Receptors
23. Pulse–Chase Analysis for Studies of MHC Class II Biosynthesis, Maturation, and Peptide Loading
24. Studying MHC Class II Peptide Loading and Editing In Vitro
25. Monitoring Antigen Processing for MHC Presentation via Macroautophagy
26. Identification of Human Antigen-Specific CD4+ T-Cells with Peptide–MHC Multimer Technologies
27. Production of Antigen-Specific Human CD4+ T Cell Lines and Clones
28. Monitoring Dendritic Cell Activation and Maturation
29. Studying MHC Class II Presentation of Immobilized Antigen by B Lymphocytes
30. Analyzing Antigen Recognition by Natural Killer T Cells

The second edition volume expands on the previous edition with new and updated chapters on the latest methods used for studying presentation of antigenic peptides produced in the standard processing pathways for MHC class I and II molecules. The new chapters cover topics such as biochemical and cellular approaches to study the impact of the endoplasmic reticulum aminopeptidases; techniques to monitor MHC class I synthesis and degradation; approaches to measure processing efficacy; description of different assays measuring MHC recycling; and protocols to produce MHC class II tetramers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Antigen Processing: Methods and Protocols, Second Edition is a valuable tool for both novice and expert researchers interested in studying antigen processing and venturing out further into this evolving field.

Features
• Includes cutting-edge methods and protocols
• Provides step-by-step detail essential for reproducible results
• Contains key notes and implementation advice from the experts