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128,44 €1 Introduction of SELEX and Important SELEX Variants 1
2 In Chemico Modification of Nucleotides for Better Recognition 27
3 Immobilization of Aptamers on Substrates 85
4 Characterization of Aptamer-Ligand Complexes 127
5 Utilization of Aptamers for Sample Preparation in Analytical Methods 173
6 Development of Aptamer-Based Colorimetric Analytical Methods 205
7 Enzyme-Linked Aptamer Assay (ELAA) 219
8 Development of Aptamer-Based Fluorescence Sensors 229
9 Development of Aptamer-Based Electrochemical Methods 247
10 Development of Aptamer-Based Lateral Flow Assay Methods 273
11 Development of Aptamer-Based Non-labeling Methods 301
12 Challenges of SELEX and Demerits of Aptamer-Based Methods 345
13 State of the Art and Emerging Applications 365
References 390
Index 397
Due to their high affinity, specificity, and relatively cheap and fast synthesis, aptamers and aptamer-based systems have been developed in recent years for numerous analytical and medical applications. After an introduction and common protocols for aptamer synthesis, different optimisation strategies to obtain higher affinities to target molecules are presented. Characterization of aptamer-target molecule interaction and immobilization strategies are the foundation of an in-depth discussion of method developments for all relevant applications, from colorimetric methods to lateral flow immunoassays.
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