BASIC BIOTECHNIQUES FOR BIOPROCESS AND BIOENTREPRENEURSHIP

UNIT-I: ISOLATION, SCREENING AND CULTURE MAINTENANCE
1. Isolation of microorganisms
Prof. M.K Gupta
2. Screening Strategies
Dr. Abhishek Walia
3. Identification: Morphological, Biochemical and genetic characterization
Prof. Nivedita Sharma
4. Microbial Activity and Productivity Enhancement Strategies
Dr. Shashi Kant Bhatia
5. Culture maintenance, preservation and improvement
Dr. Rakshak Kumar

UNIT-II: LABORATORY TECHNIQUES AND INSTRUMENTATION
6. Biomolecules: Types, Homogenization, Bead beater, sonication
Dr. Arshad Jawed
7. Centrifugation: Basic principle, types
Dr. Gaurav Sood
8. Spectroscopy: Basic principle types and applications
Dr. Debarati Paul
9. Protein Purification: Basic Principle and techniques
Dr. Deepak Pandey
10. Chromatography: Basic principle, types and applications
Dr. Mahesh Kumar Gupta
11. Electrophoresis: Basic principle, types and applications
Dr. Gopal Krishna Joshi

UNIT-III: GENOMIC AND PROTEOMIC ANALYSIS
12. DNA, RNA, isolation methods, primer designing, sequence submission, phylogenetic analysis
Dr. Neel Kamal Sharma
13. RDT and genetic engineering: basic of RDT method, PCR and application
Dr. Pankaj Kumar
14. Protein sequence analysis
Dr. Abhishek Chaudhary
15. Computational strategies and tools for protein tertiary structure prediction
Dr. Raj Kumar
16. Docking strategies
Dr. Ragothaman M. Yennamalli
17. Methods of protein and drug interaction
Dr. Aurijit Sarkar

UNIT-IV: INDUSTRIALLY IMPORTANT ENZYMES
18. Enzymes and their significance in the Industrial bioprocesses to develop the ecofriendly products
Prof. Tek Chand Bhalla
19. Enzyme kinetics: Industrially important enzymes
Dr. Kumar Sandeep
20. Industrial enzymes: Basic information, Assay and application
Prof. P.S. Panesar

UNIT-V: TECHNIQUES IN PROCESS DEVELOPMENT
21. Basics of Fermentation
Prof. Arvind Kumar Bhatt
22. Sterilization
Dr. Gunjan Goel
23. Scale-up: Lab to Commercial Scale
Dr. Krunal Dholiya
24. Bioprocess: Control, management and Biosafety issues
Dr. Subhasish Dutta
25. Demonstration and Industrial Scaleup
Dr. Govindarajan Ramadoss
26. Downstream Processing of biotechnology products
Dr. Dattatray Bedade
27. Waste Management and Environment
Prof. Paulraj Rajamani

UNIT-VI : FEASIBILITY, MARKETING AND BIOBUSINESS
28. Bio-business opportunities
Dr. Ashutosh Gupta
29. Feasibility analysis and Business Plan
Dr. Ashok Kumar Bansal
30. Patenting, IPR and Regulatory Issues
Dr. Sarita Devi
31.Commercialization and Technology Transfers of Bioprocess
Dr. Pradeep Kumar
32. Bio-product marketing strategies
Dr. Ravi Kant Bhatia

• Offers guidance for readers on how to develop new microbiological and biotechnical processes
• Focuses on solutions to practical difficulties at all levels, creating a thorough understanding of bioprocess development, commercialization, technology transfer, and more
• Provides multifaceted coverage, with industry insights from leaders in the field
• Gives solutions to practical difficulties at all levels, i.e., Lab, Scale-Up and the Commercial stage

Authors
• Arvind Kumar Bhatt, Department of Biotechnology, Himachal Pradesh University, India.
• Ravi Kant Bhatia, Department of Biotechnology, Himachal Pradesh University, India.
• Tek Chand Bhalla, Department of Biotechnology, Himachal Pradesh University, India