BIOLOGICAL INVASIONS IN SOUTH AFRICA

• The Simplest Protocol for Rapid and Long-Term Culture of Primary Epidermal Keratinocytes from Human and Mouse
• Dermal-Epidermal Separation by Heat
• Dermal-Epidermal Separation by Enzyme
• Dermal-Epidermal Separation by Chemical
• Isolation and Characterization of Extracellular Vesicles from Keratinocyte Cultures
• Competitive Repopulation Assay of Long-Term Epidermal Stem Cell Regeneration Potential
• Quantification of Melanosome Transfer Using Immunofluorescence Microscopy and Automated Image Analysis
• Contribution of Immunohistochemistry in Revealing S100A7/JAB1 Colocalization in Psoriatic Epidermal Keratinocyte
• Digital Quantification of Epidermal Protein Expression in Paraffin-Embedded Tissue Using Immunohistochemistry
• Keratinocyte Differentiation by Flow Cytometry
• In Silico and In Vitro Considerations of Keratinocyte Nuclear Receptor Protein Structural Order for Improving Experimental Analysis
• Genetic Modification of Human Primary Keratinoctes by Lentiviral Vectors
• Generation of Knockout Human Primary Keratinocytes by CRISPR/Cas9
• In Vitro Wound Healing Assays to Investigate Epidermal Migration
• Iterative Three-Dimensional Epidermis Bioengineering and Xenografting to Assess Long-Term Regenerative Potential in Human Keratinocyte Precursor Cells
• Generation of a Full-Thickness Human Skin Equivalent on an Immunodeficient Mouse
• Biotin Identification Proteomics in Three-Dimensional Organotypic Human Skin Cultures
• Information and Statistical Analysis Pipeline for High-Throughput RNA Sequencing Data
• Cell-Extracellular Matrix Adhesion Assay
• Methods for Analysis of Keratinocyte Migration
• Scratch Wound Healing Assay
• Photoprotective Activity Assay toward Ultraviolet B in Human Keratinocytes
• Photodynamic Therapy Assay
• A Method to Prepare Claudin-Modulating Recombinant Proteins
• Human Fetal Skin Fibroblast Isolation and Expansion for Clinical Application
• Ciliated Epithelial Cell Differentiation at Air-Liquid Interface Using Commercially Available Culture Media.

This open access volume presents a comprehensive account of all aspects of biological invasions in South Africa, where research has been conducted over more than three decades, and where bold initiatives have been implemented in attempts to control invasions and to reduce their ecological, economic and social effects. It covers a broad range of themes, including history, policy development and implementation, the status of invasions of animals and plants in terrestrial, marine and freshwater environments, the development of a robust ecological theory around biological invasions, the effectiveness of management interventions, and scenarios for the future. The South African situation stands out because of the remarkable diversity of the country, and the wide range of problems encountered in its varied ecosystems, which has resulted in a disproportionate investment into both research and management. The South African experience holds many lessons for other parts of the world, and this book should be of immense value to researchers, students, managers, and policy-makers who deal with biological invasions and ecosystem management and conservation in most other regions.

Features
• Provides an encyclopedic overview of all aspects of biological invasions in South Africa, a megadiverse country that is a global leader in invasion science
• Chronicles the history of introductions, invasions, and the emergence of invasion science in South Africa, and the development of policy and legislation for dealing with invasions, spanning 150 years
• Reviews knowledge of alien species from all taxonomic groups in terrestrial, freshwater and marine environments, the determinants of invasions, their impacts, and diverse insights on management
• Assesses of the extent and effectiveness of management interventions using unique and innovative approaches
• Attempts to explore where we might be going over the next 2000 years