BIOLUMINESCENT IMAGING. METHODS AND PROTOCOLS. (SOFTCOVER)

• High-throughput Bioluminescence Imaging and Reporter Gene Assay with 3D Spheroids from Human Cell Lines
•Bioluminescence Resonance Energy Transfer (BRET) Coupled Near-Infrared Imaging of Apoptotic Cells
•High-throughput Analysis of Endocrine Disrupting Compounds using BLYES and BLYAS Bioluminescent Yeast Bioassays
•Bioluminescent Imaging of Single Bacterial Cells Using an Enhanced ilux Operon
•Bioluminescent Imaging and Tracking of Bacterial Transport in Soils
•In vivo Bioluminescent Imaging of Yersinia ruckeri Pathogenesis in Fish
•In Vivo Bioluminescent Monitoring of Parasites in BALB/c Mouse Models of Cutaneous Leishmaniasis Drug Discovery
•Multiplex Imaging of Polymicrobial Communities – Murine Models to Study Oral Microbiome Interactions
•Bioluminescence Imaging to Study Mature Biofilm Formation by Candida spp. and Antifungal Activity in vitro and in vivo
•Bioluminescence Imaging of Neuroinflammation in a Mouse Model of Parkinson’s Disease
•Continual Conscious Bioluminescent Imaging in Freely Moving Mice
•In Vivo Bioluminescent Imaging of Marburg Virus in a Rodent Model
•Continuous and Real-time In Vivo Autobioluminescent Imaging in a Mouse Model
•In vivo Tracking of Tumor-derived Bioluminescent Extracellular Vesicles in Mice
•Bioluminescence Imaging in the Chick Chorioallantoic Membrane Assay
•Application of a cybLuc Aminoluciferin for Deep Tissue Bioluminescence Imaging in Rodent Models
•The Application of Single Cell Bioluminescent Imaging to Monitor Circadian Rhythms of Individual Plant Cells.

This volume provides detailed protocols and methodologies required to perform bioluminescent imaging in multiple stages, enabling the reader to integrate this technology into their laboratory-based imaging experiments. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Bioluminescent Imaging: Methods and Protocols to ensure successful results in the further study of this vital field.

Features
• Includes cutting-edge methods and protocols
• Provides step-by-step details essential for reproducible results
• Contains key notes and implementation advice from the experts