CHEMICAL LIGATION: TOOLS FOR BIOMOLECULE SYNTHESIS AND MODIFICATION

1 Introduction to Chemical Ligation Reactions 1
2 Protein Chemical Synthesis by SEA Ligation 89
3 Development of Serine/Threonine Ligation and Its Applications 125
4 Synthesis of Proteins by Native Chemical Ligation–Desulfurization Strategies 161
5 Synthesis of Chemokines by Chemical Ligation 223
6 Chemical Synthesis of Glycoproteins by the Thioester Method 251
7 Membrane Proteins: Chemical Synthesis and Ligation 269
8 Chemoselective Modification of Proteins 285
9 Stable, Versatile Conjugation Chemistries for Modifying Aldehyde-Containing Biomolecules 339
10 Thioamide Labeling of Proteins through a Combination of Semisynthetic Methods 355
11 Macrocyclic Organo-Peptide Hybrids by Intein-Mediated Ligation: Synthesis and Applications 391
12 Protein Ligation by HINT Domains 421
13 Chemical Ligation for Molecular Imaging 447
14 Native Chemical Ligation in Structural Biology 485

Presenting a wide array of information on chemical ligation – one of the more powerful tools for protein and peptide synthesis – this book helps readers understand key methodologies and applications that protein therapeutic synthesis, drug discovery, and molecular imaging.

• Moves from fundamental to applied aspects, so that novice readers can follow the entire book and apply these reactions in the lab
• Presents a wide array of information on chemical ligation reactions, otherwise scattered across the literature, into one source
• Features comprehensive and multidisciplinary coverage that goes from basics to advanced topics
• Helps researchers choose the right chemical ligation technique for their needs

Author Information
• Luca D. D'Andrea, PhD, is Research Scientist at the Institute of Biostructures and Bioimaging, CNR Naples, Italy. His scientific interests are in the field of peptide and protein chemistry. His research activity focuses on design, synthesis, and structural characterization of peptide/proteins as therapeutic/diagnostic agents.
• Alessandra Romanelli, PhD, is assistant professor of General Chemistry at Department of Pharmacy, University of Naples "Federico II", Italy. She actively works in the field of peptides and peptide-based molecules (such as peptide nucleic acids) as tools for chemical biology.