CITRUS TRISTEZA VIRUS. METHODS AND PROTOCOLS

A Short Note on Reflections and Publications on Citrus Tristeza Virus (CTV) Methodologies
A Brief Historical Account of the Family Closteroviridae
Phenotyping Biological Properties of CTV Isolates
CTV Vectors and Interactions with the Virus and Host Plants
Tissue Print and Squash Captures Real-Time RT-PCR Method for Direct Detection of Citrus Tristeza Virus (CTV) in Plant or Vector Tissues
Detection of Citrus Tristeza Virus and Co-Infecting Viroids
Assessment of Genetic Variability of Citrus Tristeza Virus by SSCP and CE-SSCP
Identification and Characterization of Resistance-Breaking (RB) Isolates of Citrus Tristeza Virus
Genotyping Citrus Tristeza Virus Isolates by Sequential Multiplex RT-PCR and Microarray Hybridization in a Lab-On-Chip Device
Rapid and Sensitive Detection of Citrus Tristeza Virus Using Reverse Transcription-Loop-Mediated Isothermal Ampli?cation (RT-LAMP) Assay
Amplification and Cloning of Large cDNA Fragments of the Citrus Tristeza Virus Genome
Bioinformatic Tools and Genome Analysis of Citrus Tristeza Virus
Analysis of Genotype Composition of Citrus Tristeza Virus Populations Using Illumina Miseq Technology
Citrus Tristeza Virus: Host RNA Silencing and Virus Counteraction
Proteomic Response of Host Plants to Citrus Tristeza Virus
Gene Expression in Citrus Plant Cells Using Helios® Gene Gun System for Particle Bombardment
Methods for Producing Transgenic Plants Resistant to CTV.

This book provides methods and clear protocols for the various technologies available to detect, characterize, and study Citrus tristeza virus (CTV), a member of the genus Closterovirus, family Closteroviridae. Thanks to the highly sensitive and specific diagnostic procedures developed, knowledge of the molecular characteristics, expression strategies, genetic variability, and epidemiology of the virus has improved significantly, as this volume reflects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Citrus Tristeza Virus: Methods and Protocols serves as an ideal guide for plant pathologists, plant virologists, molecular biologists, and graduate students interested in performing qualitative and quantitative tests as well as recently-developed diagnostic methods in order to find solutions to improve the management of this disease

Features
• Includes cutting-edge techniques
• Provides step-by-step detail essential for reproducible results
• Contains key implementation advice from the experts