1. Single-Cell RNA-Sequencing of Somatosensory Neurons
2. Preparation of Human and Rodent Spinal Cord Nuclei for Single-Nucleus Transcriptomic Analysis
3. Multiplex In Situ Hybridization of the Primate and Rodent DRG and Spinal Cord
4. Using Translating Ribosome Affinity Purification (TRAP) to Understand Cell-Specific Translatomes in Pain States
5. Ex Vivo Skin-Teased Fiber Recordings from Tibial Nerve
6. Single Unit Electrophysiological Recordings of Primary Muscle Sensory Neurons using a Novel Ex Vivo Preparation
7. Electrophysiological Recording Techniques from Human Dorsal Root Ganglion
8. Human Pluripotent Stem Cell-Derived Sensory Neurons: A New Translational Approach to Study Mechanisms of Sensitization?
9. Intraspinal Transplantation of Precursors of Cortical GABAergic Interneurons to Treat Neuropathic Pain
10. A Co-Culture System for Studying Dorsal Spinal Cord Synaptogenesis
11. Visualizing Synaptic Connectivity using Confocal and Electron Microscopy: Neuroanatomical Approaches to Define Spinal Circuits
12. Using Viral Vectors to Visualize Pain-Related Neural Circuits in Mice
13. Recording Pain-Related Brain Activity in Behaving Animals using Calcium Imaging and Miniature Microscopes
14. Optical Imaging of the Spinal Cord for the Study of Pain: From Molecules to Neural Networks
15. In Vivo Calcium Imaging of Peripheral Ganglia
16. Optogenetic Modulation of the Visceromotor Response to Reveal Visceral Pain Mechanisms
17. Use of Optogenetics for the Study of Skin-Nerve Communication
18. Channelrhodopsin-2 Assisted Circuit Mapping in the Spinal Cord Dorsal Horn
19. Production of AAVs and Injection into the Spinal Cord
20. The Use of Intraspinally Injected Chemogenetic Receptor, PSAM-GlyR, to Probe the Functional Role of Spinal Dorsal Horn Neurons
21. Measuring Mouse Somatosensory Reflexive Behaviors with High-Speed Videography, Statistical Modeling, and Machine Learning
This volume contains experimental approaches that are currently revolutionizing our understanding of the neurobiology of pain. The chapters cover many cutting-edge methods including the identification of gene expression profiles, transcriptomes or translatomes, from individual cells or defined groups of cells in rodents and primates; the electrophysiological investigation of human tissues, such as human dorsal root ganglion neurons; ways to assess modality response profiles of neurons using calcium imaging in vitro and in vivo; and somatosensory behaviors in rodents using high-speed videography and machine learning. In the Neuromethods series style, the chapters include detailed advice from specialists to obtain successful results in your laboratory.
Cutting-edge and comprehensive, Contemporary Approaches to the Study of Pain: From Molecules to Neural Networks is a valuable resource for scientists and researchers interested in making impactful contributions to our understanding of pain.