CRISPR-CAS ENZYMES (METHODS IN ENZYMOLOGY, VOL. 616)

1. Predicting and visualizing features of CRISPR–Cas systems
2. Reconstitution and biochemical characterization of ribonucleoprotein complexes in Type I-E CRISPR–Cas systems
3. Sortase-mediated fluorescent labeling of CRISPR complexes
4. Fluorescence-based methods for measuring target interference by CRISPR–Cas systems
5. Probing Cascade complex composition and stability using native mass spectrometry techniques
6. High-throughput determination of in vivo DNA sequence preferences for Cas protein binding using Library-ChIP
7. Live-cell single-particle tracking photoactivated localization microscopy of Cascade-mediated DNA surveillance
8. In vitro assembly of thermostable Csm complex in CRISPR–Cas type III/A system
9. Investigation of the cyclic oligoadenylate signaling pathway of type III CRISPR systems
10. A pipeline for characterization of novel Cas9 orthologs
11. Preparation and electroporation of Cas12a/Cpf1-guide RNA complexes for introducing large gene deletions in mouse embryonic stem cells
12. Directed evolution studies of a thermophilic Type II-C Cas9
13. Kinetic characterization of Cas9 enzymes
14. Single-molecule FRET studies of Cas9 endonuclease
15. CRISPR–Cas molecular beacons as tool for studies of assembly of CRISPR–Cas effector complexes and their interactions with DNA
16. Adapting dCas9-APEX2 for subnuclear proteomic profiling
17. CRISPR–Cas10 assisted editing of virulent staphylococcal phages
18. Reconstitution of CRISPR adaptation in vitro and its detection by PCR

• Provides the authority and expertise of leading contributors from an international board of authors
• Presents the latest release in the Methods in Enzymology series
• Updated release includes the latest information on the CRISPR-Cas Enzymes