DNA SENSORS AND INFLAMMASOMES (METHODS IN ENZYMOLOGY, VOL. 625)

1. Intercellular communication in the innate immune system through the cGAS-STING pathway
2. Analysis of human cGAS activity and structure
3. Chemical synthesis, purification, and characterization of 3'-5'-linked canonical cyclic dinucleotides (CDNs)
4. An SPR-based analysis of cGAS substrate D and steady-state M values
5. A pyrophosphatase-coupled assay to monitor the NTase activity of cGAS
6. Tracking the polymerization of DNA sensors and inflammasomes using FRET
7. Monitoring gasdermin pore formation in vitro
8. Measuring TREX1 and TREX2 exonuclease activities
9. Kinetics of DNA: Protein association and dissociation by stopped-flow spectroscopy
10. Characterization of DNA bound cyclic GMP-AMP synthase using atomic force microscopy imaging
11. Preparation of filamentous proteins for electron microscopy visualization and reconstruction
12. Cryo-EM studies of NAIP–NLRC4 inflammasomes
13. An integrative protocol for the structure determination of the mouse ASC-PYD filament
14. Protein interactions of the inflammasome adapter ASC by solution NMR
15. Tracking DNA sensor and inflammasome complexes in cells using confocal microscopy
16. Assessment of inflammasome and type I IFN responses to DNA viruses and DNA PAMPS
17. Biochemical and microscopic analysis of inflammasome complex formation
18. Comet and micronucleus assays for analyzing DNA damage and genome integrity
19. Workflows and considerations for investigating protein interactions of viral DNA sensors
20. TUBE and UbiCRest assays for elucidating polyubiquitin modifications in protein complexes

• Provides the authority and expertise of leading contributors from an international board of authors
• Presents the latest release in the Methods in Enzymology series
• Includes the latest information on DNA sensors and inflammasomes

Author
Jungsan Sohn, Johns Hopkins School of Medicine, MD, USA