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142,50 €Introduction
Principles of Light Microscopy
Fluorescence Microscopy
Fluorescence Labeling
Confocal Microscopy
Fluorescence Photobleaching and Photoactivation Techniques
Förster Resonance Energy Transfer and Fluorescence Lifetime Imaging
Multi-Photon Excitation
Single Molecule Microscopy in the Life Sciences
Super-Resolution Microscopy: Interference and Pattern Techniques
STED Microscopy
Light Sheet Fluorescence Microscopy
Appendix A: Practical Guide to Optical Alignment
Appendix B: Matrices and Images
While there are many publications on the topic written by experts for experts, this text is specifically designed to allow advanced students and researchers with no background in physics to comprehend novel fluorescence microscopy techniques.
This second edition features new chapters and a subsequent focus on super-resolution and single-molecule microscopy as well as an expanded introduction. Each chapter is written by a renowned expert in the field, and has been thoroughly revised to reflect the developments in recent years.
Author Information
Ulrich Kubitscheck is the department head of Biophysical Chemistry at the Institute of Physical and Theoretical Chemistry at the University of Bonn, Germany. Having obtained his academic degrees from the University of Bremen, Germany, he spent his career working at The Weizmann Institute of Science and the Institute of Medical Physics and Biophysics of the University of Münster, Germany, before taking up his present appointment at the University of Bonn. Professor Kubitscheck develops single molecule and light sheet imaging techniques, has authored over 80 scientific publications and has extensive experience in teaching courses on physical chemistry, biophysics and quantitative microscopy.