HUMAN PLURIPOTENT STEM CELLS: A PRACTICAL GUIDE

Aseptic Techniques
Mammalian Cell Culture Basics
Review of Biochemical Calculations
Introduction to Stem Cells
PSC Culture
Reprogramming
Differentiation
PSC Characterization
Other Considerations
Appendix I: Reagents, Medias, and Solutions
Appendix II: Common Antibodies
Appendix III: Consumable and Equipment
Appendix IV: Logs and Formas

Comprehensive coverage of the entire induced pluripotent stem cell basic work flow
Pluripotent stem cells (PSC) can divide indefinitely, self-renew, and can differentiate to functionally reconstitute almost any cell in the normal developmental pathway, given the right conditions. This comprehensive book, which was developed from a training course, covers all of the PSCs (embryonic, embryonic germ, and embryonic carcinoma) and their functions. It demonstrates the feeder-dependent and feeder-free culture of hESC and hiPSC, which will be referred to in all protocols as PSCs. It also addresses the methods commonly used to determine pluripotency, as defined by self-renewal marker expression and differentiation potential.
Human Pluripotent Stem Cells: A Practical Guide offers in-depth chapter coverage of introduction to stem cell, PSC culture, reprogramming, differentiation, PSC characterization, and more. It also includes four appendixes containing information on reagents, medias, and solutions; common antibodies; consumable and equipment; and logs and forms.

• Includes helpful tips and tricks that are normally omitted from regular research papers
• Features useful images to support the technical aspects and results visually as well as diagrammatic illustrations
• Presents specific sections (ie: reprogramming, differentiation) in a concise and easily digestible manner
• Written by experts with extensive experience in stem cell technologies

Human Pluripotent Stem Cells: A Practical Guide is an ideal text for stem cell researchers, including principal investigators, and others in university and industry settings, and for new graduate students in PSC labs.

Authors
• Uma Lakshmipathy, Director R&D, Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, USA
• Chad C. MacArthur, Staff Scientist R&D, Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, USA
• Mahalakshmi Sridharan, Scientist R&D, Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, USA
• Rene H. Quintanilla, Scientist R&D, Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, USA