HUMAN RESPIRATORY SYNCYTIAL VIRUS. METHODS AND PROTOCOLS

1. Human Respiratory Syncytial Virus: An Introduction
2. RSV Growth and Quantification by Microtitration and qRT-PCR Assays
3. Quantification of RSV Infectious Particles by Plaque Assay and Immunostaining Assay
4. Detection of RSV Antibodies in Human Plasma by Enzyme Immunoassays
5. Secretory Expression and Purification of Respiratory Syncytial Virus G and F Proteins in Human Cells
6. Development of Human Monoclonal Antibodies Against Respiratory Syncytial Virus Using a High Efficiency Human Hybridoma Technique
7. Respiratory Syncytial Virus (RSV): Neutralizing Antibody, a Correlate of Immune Protection
8. Host Factors Modulating RSV Infection: Use of Small Interfering RNAs to Probe Functional Importance
9. In Vitro Modeling of RSV Infection and Cytopathogenesis in Well-Differentiated Human Primary Airway Epithelial Cells (WD-PAECs)
10. Reverse Genetics of Respiratory Syncytial Virus
11. Use of Minigenome Systems to Study RSV Transcription
12. Screening for Host Factors Directly Interacting with RSV Protein: Microfluidics
13. A Proteomic-Based Workflow Using Purified Respiratory Syncytial Virus Particles to Identify Cellular Factors as Drug Targets
14. MicroRNA Profiling from RSV-Infected Biofluids, Whole Blood, and Tissue Samples
15. Mouse and Cotton Rat Models of Human Respiratory Syncytial Virus
16. In Vivo Assessment of Airway Function in the Mouse Model
17. Evaluation of the Adaptive Immune Response to Respiratory Syncytial Virus

This volume summarizes current techniques; ranging from protocols for virus growth, isolation, quantification and generation of recombinant RSV virus to procedures for the efficient characterization of the host immune response to RSV infection. These techniques are used in numerous laboratories around the world and are the building blocks that support the majority of RSV virus research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Humana Respiratory Syncytial Virus: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Features
• Includes cutting-edge methods and protocols
• Provides step-by-step detail essential for reproducible results
• Contains key notes and implementation advice from the experts