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153,90 ۥ Methods to produce and purify ion channels
1. Effective production of oligomeric membrane proteins by EarlyBac
2. Preparation of the Transient Receptor Potential Vanilloid 2 (TRPV2) channel for structural studies
3. Production and Purification of TRPV2 and TRPV5 for Cryo-Electron Microscopy Studies
4. Sample Preparation of the Human TRPA1 Ion Channel for Cryo-EM Studies
5. Expression and Purification of the Cardiac Sodium Channel NaV1.5 for Cryo-EM Structure Determination
6. Structural determination of human Nav1.4 and Nav1.7 using single particle cryo-electron microscopy
7. Production and Purification of ATP-Sensitive Potassium Channel Particles for Cryo-Electron Microscopy
8. Production of K2P2.1 (TREK-1) for structural studies
9. Purification of a native nicotinic receptor
10. SARS-CoV-2 3a expression, purification, and reconstitution into lipid nanodiscs
• Optical methods for probing and controlling channel function
11. Studying Ion Channel Conformation Dynamics by Encoding Coumarin as Unnatural Amino Acid
12. Fluorescence lifetime measurement of absolute membrane potential
13. Fluorescent Toxins as Ion Channel Activity Sensors
14. Probing Ion Channel Macromolecular Interactions using Fluorescence Resonance Energy Transfer
15. Manipulation of Ion Channel Gating with Photoswitchable Tweezers
16. Counting Subunits in Kv Channel Complexes
• Provides the authority and expertise of leading contributors from an international board of authors
• Presents the latest release in the Methods in Enzymology series
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