MANUAL OF CLINICAL MICROBIOLOGY, 2 VOLS. 12TH EDITION

• section I Diagnostic Strategies and General Topics / 1
1 Introduction to the 12th Edition of the Manual of Clinical Microbiology / 3
2 Laboratory Accreditation and Compliance / 5
3 Microscopy / 16
4 Laboratory Detection of Bacteremia and Fungemia / 28
5 Systems for Identification of Bacteria and Fungi / 45
6 Automation and Design of the Clinical Microbiology Laboratory / 72
7 Molecular Microbiology / 86
8 Immunoassays for the Diagnosis of Infectious Diseases / 124
9 Prevention of Health Care-Associated Infections / 139
10 Investigation of Disease Outbreaks / 155
11 Molecular Epidemiology / 167
12 Procedures for the Storage of Microorganisms / 197
13 Prevention of Laboratory-Acquired Infections / 206
14 Disinfection and Sterilization / 224
15 Biothreat Agents / 243
16 The Human Microbiome / 254
17 Microbial Genomics and Pathogen Discovery / 269
• section II. Bacteriology / 284
18 Taxonomy of Bacteria and Archaea / 287
19 Specimen Collection, Transport, and Processing: Bacteriology / 302
20 Reagents, Stains, and Media: Bacteriology / 331
Gram-Positive Cocci
21 General Approaches to Identification of Aerobic Gram-Positive Cocci / 362
22 Staphylococcus, Micrococcus, and Other Catalase-Positive Cocci / 367
23 Streptococcus / 399
24 Enterococcus / 418
25 Aerococcus, Abiotrophia, and Other Aerobic Catalase-Negative, Gram-Positive Cocci / 436
Gram-Positive Rods
26 General Approaches to the Identification of Aerobic Gram-Positive Rods / 451
27 Bacillus and Other Aerobic Endospore-Forming Bacteria / 455
28 Listeria and Erysipelothrix / 477
29 Coryneform Gram-Positive Rods / 488
30 Nocardia, Rhodococcus, Gordonia, Actinomadura, Streptomyces, and Other Aerobic Actinomycetes / 525
31 Mycobacterium: General Characteristics, Laboratory Detection, and Staining Procedures / 558
32 Mycobacterium tuberculosis Complex / 576
33 Mycobacterium: Laboratory Characteristics of Slowly Growing Mycobacteria Other than Mycobacterium tuberculosis / 595
34 Mycobacterium: Clinical and Laboratory Characteristics of Rapidly Growing Mycobacteria / 612
Gram-Negative Bacteria
35 Approaches to the Identification of Aerobic Gram-Negative Bacteria / 630
36 Neisseria / 640
37 Aggregatibacter, Capnocytophaga, Eikenella, Kingella, Pasteurella, and Other Fastidious or Rarely Encountered Gram-Negative Rods / 656
38 Haemophilus / 670
39 Escherichia, Shigella, and Salmonella / 688
40 Klebsiella and Selected Enterobacterales / 724
41 Yersinia / 751
42 Aeromonas / 765
43 Vibrio and Related Organisms / 775
44 Pseudomonas / 787
45 Burkholderia, Stenotrophomonas, Ralstonia, Cupriavidus, Pandoraea, Brevundimonas, Comamonas, Delftia, and Acidovorax / 807
46 Acinetobacter, Chryseobacterium, Moraxella, and Other Nonfermentative Gram-Negative Rods / 829
47 Bordetella and Related Genera / 858
48 Francisella / 871
49 Brucella / 883
50 Bartonella / 893
51 Legionella / 905
Anaerobic Bacteria
52 Approaches to the Identification of Anaerobic Bacteria / 921
53 Peptostreptococcus, Finegoldia, Anaerococcus, Peptoniphilus, Parvimonas, Murdochiella, Veillonella, and Other Anaerobic Cocci / 925
54 Actinomyces, Lactobacillus, Cutibacterium, and Other Non-Spore-Forming Anaerobic Gram-Positive Rods / 938
55 Clostridium and Clostridioides / 968
56 Bacteroides, Porphyromonas, Prevotella, Fusobacterium, and Other Anaerobic Gram-Negative Rods / 995
Curved and Spiral-Shaped Gram-Negative Rods
57 Algorithms for Identification of Curved and Spiral-Shaped Gram-Negative Rods / 1024
58 Campylobacter and Arcobacter / 1028
59 Helicobacter / 1044
60 Leptospira / 1058
61 Borrelia / 1066
62 Treponema and Brachyspira: Human Host-Associated Spirochetes / 1083
Mycoplasmas and Obligate Intracellular Bacteria
63 General Approaches to Identification of Mycoplasma, Ureaplasma, and Obligate Intracellular Bacteria / 1109
64 Mycoplasma and Ureaplasma / 1117
65 Chlamydia / 1137
66 Rickettsia and Orientia / 1149
67 Ehrlichia, Anaplasma, and Related Intracellular Bacteria / 1163
68 Coxiella / 1180
69 Tropheryma whipplei / 1189
• section III. Antibacterial Agents and Susceptibility Test Methods / 1199
70 Antibacterial Agents / 1201
71 Mechanisms of Resistance to Antibacterial Agents / 1242
72 Susceptibility Test Methods: General Considerations / 1277
73 Antimicrobial Susceptibility Test Methods: Dilution and Disk Diffusion Methods / 1284
74 Antimicrobial Susceptibility Testing Systems / 1300
75 Special Phenotypic Methods for Detecting Antibacterial Resistance / 1316
76 Susceptibility Test Methods: Fastidious Bacteria / 1348
77 Susceptibility Test Methods: Anaerobic Bacteria / 1377
78 Susceptibility Test Methods: Mycobacteria, Nocardia, and Other Actinomycetes / 1398
79 Molecular Detection of Antibacterial Drug
Resistance / 1420

Volume 2
• section IV. Virology / 1432
General
80 Taxonomy and Classification of Viruses / 1435
81 Specimen Collection, Transport, and Processing: Virology / 1446
82 Reagents, Stains, Media, and Cell Cultures: Virology / 1462
83 Algorithms for Detection and Identification
of Viruses / 1472
RNA Viruses
84 Human Immunodeficiency Viruses / 1477
85 Human T-Cell Lymphotropic Viruses / 1498
86 Influenza Viruses / 1510
87 Parainfluenza and Mumps Viruses / 1528
88 Respiratory Syncytial Virus and Human Metapneumovirus / 1541
89 Measles and Rubella Viruses / 1560
90 Enteroviruses and Parechoviruses / 1576
91 Rhinoviruses / 1592
92 Coronaviruses / 1606
93 Hepatitis A and E Viruses / 1625
94 Hepatitis C Virus / 1640
95 Gastroenteritis Viruses / 1656
96 Rabies Lyssavirus / 1674
97 Arboviruses / 1685
98 Hantaviruses / 1706
99 Arenaviruses and Filoviruses / 1719
DNA Viruses
100 Herpes Simplex Viruses and Herpes B Virus / 1740
101 Varicella-Zoster Virus / 1757
102 Human Cytomegalovirus / 1770
103 Epstein-Barr Virus / 1797
104 Human Herpesviruses 6A, 6B, and 7 / 1814
105 Human Herpesvirus 8 / 1826
106 Adenoviruses / 1831
107 Human Papillomaviruses / 1847
108 Human Polyomaviruses / 1863
109 Parvovirus B19 and Bocaviruses / 1880
110 Poxviruses / 1891
111 Hepatitis B and D Viruses / 1905
Subviral Agents
112 Prion Diseases / 1923
• section V Antiviral Agents and Susceptibility Test Methods / 1935
113 Antiviral Agents / 1937
114 Mechanisms of Resistance to Antiviral Agents / 1962
115 Susceptibility Test Methods: Viruses / 1985
• section VI Mycology / 2005
General
116 Taxonomy, Classification, and Nomenclature of Fungi / 2007
117 Specimen Collection, Transport, and Processing: Mycology / 2016
118 Reagents, Stains, and Media: Mycology / 2025
119 General Approaches for Direct Detection and Identification of Fungi / 2035
Fungi
120 Candida, Cryptococcus, and Other Yeasts of Medical Importance / 2056
121 Pneumocystis / 2087
122 Aspergillus, Talaromyces, and Penicillium / 2103
123 Fusarium and Other Opportunistic Hyaline Fungi / 2132
124 Agents of Systemic and Subcutaneous Mucormycosis and Entomophthoromycosis / 2163
125 Histoplasma, Blastomyces, Coccidioides, Paracoccidioides, and Other Dimorphic Fungi Causing Systemic Mycoses / 2187
126 Trichophyton, Microsporum, Epidermophyton, and Agents of Superficial Mycoses / 2208
127 Curvularia, Exophiala, Scedosporium, Sporothrix, and Other Melanized Fungi / 2234
128 Fungi Causing Eumycotic Mycetoma / 2261
129 Mycotoxins / 2278
130 Lacazia, Lagenidium, Pythium, and Rhinosporidium / 2291
131 Microsporidia / 2305
• section VII. Antifungal Agents and Susceptibility Test Methods / 2317
132 Antifungal Agents / 2319
133 Mechanisms of Resistance to Antifungal Agents / 2334
134 Susceptibility Test Methods: Yeasts and Filamentous Fungi / 2351
• section VIII. Parasitology / 2377
General
135 Taxonomy and Classification of Human Eukaryotic Parasites / 2379
136 Specimen Collection, Transport, and Processing: Parasitology / 2389
137 Reagents, Stains, and Media: Parasitology / 2406
138 General Approaches for Detection and Identification of Parasites / 2414
Parasites
139 Plasmodium and Babesia / 2438
140 Leishmania and Trypanosoma / 2458
141 Toxoplasma / 2473
142 Pathogenic and Opportunistic Free-Living Amebae / 2486
143 Intestinal and Urogenital Amebae, Flagellates, and Ciliates / 2497
144 Cystoisospora, Cyclospora, and Sarcocystis / 2526
145 Cryptosporidium / 2536
146 Nematodes / 2551
147 Filarial Nematodes / 2570
148 Cestodes / 2582
149 Trematodes / 2590
150 Less Common Helminths / 2606
151 Arthropods of Medical Importance / 2620
• section IX. Antiparasitic Agents and Susceptibility Test Methods / 2643
152 Antiparasitic Agents / 2645
153 Mechanisms of Resistance to Antiparasitic Agents / 2669
154 Susceptibility Test Methods: Parasites / 2682

Revised by a collaborative, international, interdisciplinary team of editors and authors, this edition includes the latest applications of genomics and proteomics and is filled with current findings regarding infectious agents, leading-edge diagnostic methods, laboratory practices, and safety guidelines. This edition also features three new chapters on accreditation, Mycobacterium tuberculosis complex, and human herpesvirus 8. This seminal reference of microbiology continues to set the standard for state-of-the-science laboratory practice as the most authoritative reference in the field of microbiology.

Author
Dr. Karen Carroll is a professor of pathology at the Johns Hopkins University School of Medicine.