METHODS IN ENZYMOLOGY. INTERMEDIATE FILAMENT ASSOCIATED PROTEINS. VOL. 569

• Continues the legacy of this premier serial with quality chapters authored by leaders in the field
• Covers research methods in intermediate filament associated proteins and contains sections on such topics as lamin-associated proteins, intermediate filament-associated proteins and plakin, and other cytoskeletal cross-linkers

Authors
• Katherine L. Wilson, Department of Cell Biology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
• Arnoud Sonnenberg, Department of Cell Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands.

Contents

Part I: Identification and Biochemical Analysis of Lamin-Associated Proteins
• Chapter One: BioID Identification of Lamin-Associated Proteins
• Chapter Two: Proximity-Dependent Biotin Identification (BioID) in Dictyostelium Amoebae
• Chapter Three: Purification and Structural Analysis of LEM-Domain Proteins
• Chapter Four: Purification and Structural Analysis of SUN and KASH Domain Proteins
• Chapter Five: Purification of Lamins and Soluble Fragments of NETs
• Chapter Six: Simple Separation of Functionally Distinct Populations of Lamin-Binding Proteins
Part II: General Methods for Analysis of Intermediate Filament-Associated Proteins
• Chapter Seven: Development of a Novel Green Fluorescent Protein-Based Binding Assay to Study the Association of Plakins with Intermediate Filament Proteins
• Chapter Eight: Functional Analysis of Keratin-Associated Proteins in Intestinal Epithelia: Heat-Shock Protein Chaperoning and Kinase Rescue
• Chapter Nine: Purification of Protein Chaperones and Their Functional Assays with Intermediate Filaments
• Chapter Ten: Purification and Structural Analysis of Plectin and BPAG1e
• Chapter Eleven: Purification and Structural Analysis of Desmoplakin
• Chapter Twelve: Degradation of the Intermediate Filament Family by Gigaxonin
Part III: Functional and Genetic Analysis of the Plakin and Other Cytoskeletal Cross-Linkers
• Chapter Thirteen: Functional and Genetic Analysis of Plectin in Skin and Muscle
• Chapter Fourteen: Functional and Genetic Analysis of Epiplakin in Epithelial Cells
• Chapter Fifteen: In Vitro Model of the Epidermis: Connecting Protein Function to 3D Structure
• Chapter Sixteen: Functional Analysis of Periplakin and Envoplakin, Cytoskeletal Linkers, and Cornified Envelope Precursor Proteins
• Chapter Seventeen: Microtubule-Actin Cross-Linking Factor 1: Domains, Interaction Partners, and Tissue-Specific Functions
• Chapter Eighteen: Functional and Genetic Analysis of Neuronal Isoforms of BPAG1
• Chapter Nineteen: Functional and Genetic Analysis of Spectraplakins in Drosophila
• Chapter Twenty: Functional and Genetic Analysis of VAB-10 Spectraplakin in Caenorhabditis elegans
Part IV: Functional and Genetic Analysis of Lamin-Associated Proteins
• Chapter Twenty-One: Tagged Chromosomal Insertion Site System: A Method to Study Lamina-Associated Chromatin
• Chapter Twenty-Two: Lamin-Binding Proteins in Caenorhabditis elegans
• Chapter Twenty-Three: Pathologically Relevant Prelamin A Interactions with Transcription Factors
• Chapter Twenty-Four: MCLIP Detection of Novel Protein–Protein Interactions at the Nuclear Envelope
• Chapter Twenty-Five: Preparation of the Human Cytomegalovirus Nuclear Egress Complex and Associated Proteins
Author Index
Subject Index