METHODS IN ENZYMOLOGY VOL. 520. G PROTEIN COUPLED RECEPTORS

Expression of GPCRs in Pichia pastoris for structural studies

Christoph Krettler, Christoph Reinhart and Carville G. Bevans

Comformational Ensemble View of G Protein-Coupled Receptors and The Effect of Mutations and Ligand Binding

Ravinder Abrol, Soo-Kyung Kim, Jenelle K. Bray, Bartosz Trzaskowski, and William A. Goddard III


Structural Evolution of G-Protein-Coupled Receptors: A Sequence Space Approach

Jean-Michel Bécu, Julien Pelé, Patrice Rodien, Hervé Abdi and Marie Chabbert


Directed Evolution of G Protein-Coupled Receptors for High Functional Expression and Detergent Stability

Karola M. Schlinkmann and Andreas Plückthun


The Role of Hydrophobic Amino Acids in the Structure and Function of the Rhodopsin Family of G Protein-Coupled Receptors

Gianluigi Caltabiano, Angel González, Arnau Cordomí, Mercedes Campillo, and Leonardo Pardo


Structure of ß-Adrenergic Receptors

Florian Brueckner, Chayne L. Piscitelli, Ching-Ju Tsai, Jörg Standfuss, Xavier Deupi and Gebhard F.X. Schertler


Advances in Methods to Characterize Ligand-Induced Ionic Lock and Rotamer Toggle Molecular Switch in G-Protein Coupled Receptors

Xiang-Qun Xie and Ananda Chowdhury


Crystallogenesis of Adenosine A2A Receptor – T4 Lysozyme Fusion Protein: A Practical Route for the Structure

Yashwanth Ashok, Rahul T. Nanekar & Veli-Pekka Jaakola


Probing GPCR Structure: Adenosine and P2Y Nucleotide Receptors

Kenneth A. Jacobson, Stefano Costanzi, and Francesca Deflorian


Strategies for Studying the Ligand Binding Site of GPCRs: Photoaffinity Labeling of the VPAC1 Receptor, a Prototype of Class B GPCRs

Alain Couvineau, Yossan-Var Tan, Emilie Ceraudo and Marc Laburthe


Expression of Mammalian G Protein-Coupled Receptors in Caenorhabditis Elegans

Beata Jastrzebska, David Salom, Hui Jin, Pengxiu Cao, Wenyu Sun, Krzysztof Palczewski, and Zhaoyang Feng


Expression, Purification and Structural Analysis of Intracellular C-Termini from Metabotropic Glutamate Receptors

Angela Seebahn, Heinrich Sticht and Ralf Enz


Unnatural Amino Acid Mutagenesis of GPCRs Using Amber Codon Suppression and Bioorthogonal Labeling

Thomas Huber, Saranga Naganathan, He Tian, Shixin Ye, and Thomas P. Sakmar


Mapping a Ligand Binding Site Using Genetically-Encoded Photoactivatable Crosslinkers

Amy Grunbeck, Thomas Huber, Thomas P. Sakmar


Alternative mRNA Splicing of G Protein-Coulped Receptors

Danijela Markovic


Functional Residues of the CB1 Cannabinoid Receptor

Joong-Youn Shim and Lea Padgett

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers G protein coupled receptors.

Features:
Continues the legacy of this premier serial with quality chapters authored by leaders in the field
Covers G protein coupled receptors
Contains chapters on such topics as post-translation modification of GPCR in relationship to biased agonism, structure-based virtual screening, and GPCR oligomerization in the brain