MOLECULAR BIOTECHNOLOGY. PRINCIPLES AND APPLICATIONS OF RECOMBINANT DNA. 5TH EDITION

The Development of Molecular Biotechnology 1
Emergence of Molecular Biotechnology 1
Recombinant DNA Technology 3
Commercialization of Molecular Biotechnology 4
Concerns and Consequences 7
SUMMARY9
REFERENCES 9
REVIEW
QUESTIONS10
Fundamental Technologies 11
Molecular Cloning 11
Genome Engineering using
CRISPR Technology 32
Polymerase Chain Reaction 35
Chemical Synthesis of DNA 42
DNA Sequencing Technologies 50
Sequencing Whole Genomes 59
Genomics 64
SUMMARY87
REFERENCES 89
REVIEW
QUESTIONS91
Production of Recombinant Proteins 93
Protein Production in Prokaryotic Hosts 93
Heterologous Protein Production in Eukaryotic Cells 120
Protein Engineering 153
SUMMARY171
REFERENCES 173
REVIEW
QUESTIONS175
Molecular Diagnostics 177
Immunological Approaches to Detect Protein Biomarkers 178
DNA-Based Diagnostic Approaches 193
Detecting RNA Signatures of Disease 215
Biofluorescent and Bioluminescent Systems 219
SUMMARY224
REFERENCES 225
REVIEW
QUESTIONS227
Protein Therapeutics 229
Pharmaceuticals 230
Enzymes 240
Lactic Acid Bacteria 255
Recombinant Antibodies 261
SUMMARY292
REFERENCES 292
REVIEW
QUESTIONS295
Nucleic Acids as Therapeutic Agents 297
Targeting Specific mRNA and DNA Sequences299
Viral Delivery Systems 319
Nonviral Delivery Systems 325
Gene Therapy 335
SUMMARY338
REFERENCES 339
REVIEW
QUESTIONS341
Vaccines 343
Vaccination 343
Current and Future Vaccines 345
Subunit Vaccines 347
Peptide Vaccines 359
Genetic Immunization: DNA Vaccines 363
Attenuated Vaccines 372
Vector Vaccines 378
Monoclonal Antibody Passive Immunity 396
SUMMARY397
REFERENCES 398
REVIEW
QUESTIONS400
Industrial and Environmental Uses of
Recombinant Microorganisms 403
Restriction Endonucleases 403
Small Biological Molecules 405
Microbial Degradation of Xenobiotics 434
Utilization of Starch and Sugars 445
Utilization of Cellulose and Hemicellulose 454
Lipids from Cyanobacteria 467
Hydrogen Production 468
SUMMARY470
REFERENCES 471
REVIEW
QUESTIONS474
Large-Scale Production of Proteins from
Recombinant Microorganisms 475
Principles of Microbial Growth 476
Maximizing The Efficiency of The Fermentation Process 481
Bioreactors 491
Typical Large-Scale Fermentation Systems 494
Harvesting Microbial Cells 501
Disrupting Microbial Cells 502
Downstream Processing 504
Large-Scale Production of Plasmid DNA 508
SUMMARY 511
REFERENCES 512
REVIEW QUESTIONS 514
10 Genetic Engineering of Plants: Methodology 515
Plant Transformation with the Ti Plasmid of
A. Tumefaciens 516
Ti Plasmid-Derived Vector Systems 522
Microprojectile Bombardment 526
Chloroplast Engineering 527
Use of Reporter Genes in Transformed Plant Cells 532
Manipulation of Gene Expression in Plants 533
Production of Marker-Free Transgenic Plants 542
SUMMARY 546
REFERENCES 547
REVIEW QUESTIONS 549
11 Transgenic Plants 551
Insect Resistance 551
Virus Resistance 570
Herbicide Resistance 578
Fungus and Bacterium Resistance 583
Salt and Drought Stress 588
Fruit Ripening and Flower Wilting 592
Modification of Plant Nutritional Content 594
Modification of Food Plant Taste and Appearance 603
Plants as Bioreactors 608
Eddible Vaccines 611
Plant Yield 615
SUMMARY619
REFERENCES 620
REVIEW
QUESTIONS624
12 Transgenic Animals 625
Transgenic Mice: Methodology 626
Transgenic Mice: Applications 644
Transgenic Livestock 656
Transgenic Poultry 669
Transgenic Fish 673
SUMMARY 676
REFERENCES 676
REVIEW QUESTIONS 678
13 Molecular Biotechnology and Society 679
Development of Guidelines for Recombinant
DNA Research 680
Deliberate Release of Genetically Modified
Microorganisms 682
Regulation of Genetically Modified Foods 684
Societal Concerns About Genetically Modified Foods 692
Regulation and Safety of Medical Products of
Biotechnology 706
Patenting Biotechnology 714
SUMMARY721
REFERENCES 722
REVIEW
QUESTIONS724
Amino Acids of Proteins and Their Designations 725
Index 727

Since 1994, Molecular Biotechnology: Principles and Applications of Recombinant DNA has introduced students to the fast-changing world of molecular biotechnology. With each revision, the authors have extensively updated the book to keep pace with the many new techniques in gene isolation and amplification, nucleic acid synthesis and sequencing, gene editing, and their applications to biotechnology. In this edition, authors Bernard R. Glick and Cheryl L. Patten have continued that tradition, but have also overhauled the book’s organization to

• Detail fundamental molecular biology methods and recombinant protein engineering techniques, which provides students with a solid scientific basis for the rest of the book.
• Present the processes of molecular biotechnology and its successes in medicine, bioremediation, raw material production, biofuels, and agriculture.
• Examine the intersection of molecular biotechnology and society, including regulation, patents, and controversies around genetically modified products.

Filled with engaging figures that strongly support the explanations in the text, Molecular Biotechnology: Principles and Applications of Recombinant DNA presents difficult scientific concepts and technically challenging methods in clear, crisp prose.
This excellent textbook is ideal for undergraduate and graduate courses in introductory biotechnology, as well as, courses dedicated to medical, agricultural, environmental, and industrial biotechnology applications.