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68,17 €Preface
Acknowledgments
Scheduling and Interrelationships of Exercises
• Chapter 1. History of Plant Cell Culture
• Chapter 2. Setup of a Tissue Culture Laboratory
• Chapter 3. Media Components and Preparation
• Chapter 4. Explant Preparation
• Chapter 5. Contamination
• Chapter 6. Callus Induction
• Chapter 7. Regeneration and Morphogenesis
• Chapter 8. Woody Shrubs and Trees
• Chapter 9. Haploid Plants from Anther Culture
• Chapter 10. Embryo Rescue
• Chapter 11. Meristem Culture for Virus-Free Plants
• Chapter 12. In Vitro Propagation for Commercial Production of Ornamentals
• Chapter 13. Protoplast Isolation and Fusion
• Chapter 14. Agrobacterium-Mediated Transformation of Plants
Appendix I: Useful Measurements
Appendix II: Review of Solution Preparation
Appendix III: List of Suppliers
Appendix IV: Common Plant Tissue Culture Terms
Index
Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples.
The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists.
Features:
• Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems
• Includes detailed drawings that complement both introductions and experiments
• Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement
• Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology
• Fully updated literature
Author
Roberta H. Smith, Emeritus Professor, Department of Horticulture, Vegetable Crops Improvement Center, Texas A&M University, College Station, Texas, U.S.A.