RADIATION CYTOGENETICS. METHODS AND PROTOCOLS

Human Lymphocyte Metaphase Chromosome Preparation for Radiation-Induced Chromosome Aberration Analysis
Animal Lymphocyte Metaphase Chromosome Preparation
Micronuclei Formation Analysis after Ionizing Radiation
G1 Premature Chromosome Condensation (PCC) Assay
G2 Chromosomal Radiosensitivity Assay for Testing Individual Radiation Sensitivity
G2 Premature Chromosome Condensation/Chromosome Aberrations Assay: Drug-Induced Premature Chromosome Condensation (PCC) Protocols and Cytogenetic Approaches in Mitotic Chromosome and Interphase Chromatin for Radiation Biology
Sister Chromatid Exchange as a Genotoxic Stress Marker
DNA Damage Focus Formation Assay
Nuclear Foci Assays in Live Cells
In Situ DNA Damaging Foci Analysis on Metaphase Chromosomes
PNA Telomere and Centromere FISH Staining for Accurate Analysis of Radiation-Induced Chromosomal Aberrations
Modified PNA Telomere and Centromere FISH Protocols
Directional Genomic Hybridization (DGH) for Detection of Intra-Chromosomal Rearrangements
Reciprocal Translocation Analysis with Whole Chromosome Painting for FISH
Analysis of Radiation-Induced Chromosome Exchanges Using Combinatorial Chromosome Painting.

This book comprises a collection of chapters describing topics from traditional radiation cytogenetic analysis methods to the modern fluorescence based-analysis and high throughput automatic analysis methods. Cytogenetic analysis is important not only in order to understand radiation-induced DNA damage in cells, but it is also a standard biological method to estimate human radiation exposure in the radiation accidents. Therefore, we prepared a series of chapters not only covering biological aspects but also exploring radiation physics and radiation protection. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and equipment, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Radiation Cytogenetics: Methods and Protocols serves both novices working on their first experiments as well as experienced researchers searching for tried-and-true techniques to adapt for their own laboratory.

Features
• Includes cutting-edge techniques
• Provides step-by-step detail essential for reproducible results
• Contains key implementation advice from the experts