RNA RECOGNITION, VOL 623

1. Preparation of cooperative RNA recognition complexes for crystallographic structural studies
2. Methods for thermal denaturation studies of nucleic acids in complex with fluorogenic dyes
3. Identifying and validating small molecules interacting with RNA (SMIRNAs)
4. Dynamic combinatorial chemistry as a rapid method for discovering sequence-selective RNA-binding compounds
5. A click chemistry assay to identify natural product ligands for pre-microRNAs
6. Differentiation and classification of RNA motifs using small molecule-based pattern recognition
7. Ligand-observed NMR techniques to probe RNA-small molecule interactions
8. Synthetic bPNAs as allosteric triggers of hammerhead ribozyme catalysis
9. Using Rosetta for RNA homology modeling
10. Monitoring co-transcriptional folding of riboswitches through helicase unwinding
11. Strategies for understanding RNA recognition by X-ray and NMR methods
12. Sensitive detection of structural features and rearrangements in long, structured RNA molecules
13. A fluorescent aminosugar to rapidly screen and study RNA binders
14. The use of electrospray ionization mass spectrometry to monitor RNA-ligand interactions
15. Design of RNA-targeting macrocyclic peptides
16. Molecular recognition of HIV-1 RNAs with branched peptides
17. Sequence-specific recognition of structured RNA by triplex-forming peptide nucleic acids
18. Evaluating riboswitch optimality

RNA Recognition, Volume 623, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This updated volume covers a variety of topics, including The Preparation of cooperative RNA recognition complexes for crystallographic structural studies, Methods for thermal denaturation studies of fluorogenic aptamers, Dynamic combinatorial chemistry as a rapid, fragment-based approach to RNA-targeted compound discovery, Using a click chemistry assay to identify natural product ligands for pre-microRNAs, Lessons from exploration of chemical and structural small molecule:RNA space, Using ligand-observed NMR to study RNA-small molecule interactions, and much more.

Features:
• Provides the authority and expertise of leading contributors from an international board of authors
• Presents the latest release in the Methods in Enzymology series
• Includes the latest information on RNA Recognition