SCREENING THE SINGLE EUPLOID EMBRYO. MOLECULAR GENETICS IN REPRODUCTIVE MEDICINE

Intended for readers with a background in fertility medicine as well as those less familiar with IVF, this comprehensive work presents an update on preimplantation genetic testing to enable single embryo transfer (SET). An international cast of contributors explains the treatment sequence—from ovulation induction to luteal support—aiming to transfer only one euploid embryo. Applications of molecular techniques for gamete and embryo assessment are fully detailed, with a focus on the strengths and limitations of each. In addition, expert commentary is shared across a range of regulatory challenges associated with embryo screening and cryopreservation. As access to advanced reproductive technology increases against a sharper background of healthcare reform, clinicians, economists, bioethicists and legislators alike will find this new volume relevant and highly accessible.

Feature:
- Provides explanation of each step of the process -- from ovulation induction to embryo transfer
- Maximizes reader insights into the value of transferring a single embryo and explores ethical and regulatory issues in single embryo IVF transfer
- Details and assesses the molecular techniques for embryo evaluation

Author
Eric Scott Sills received his undergraduate degree from Vanderbilt and holds a PhD (molecular biology) from London’s University of Westminster. Dr. Sills was awarded the MD degree from University of Tennessee in 1992. After a gynecology residency at NYU Downtown Hospital, he completed his sub-specialty training in reproductive endocrinology at Cornell University. Having authored more than 100 peer-reviewed publications, Dr. Sills served as editor-in-chief for the Journal of Experimental & Clinical Assisted Reproduction from 2004–2014. He is board-certified by the American Board of Obstetrics and Gynecology, and is a Fellow of the American College of Obstetricians and Gynecologists, American College of Surgeons, Royal College of Physicians (Ireland), and Society of Biology (UK). At present, Dr. Sills is Medical Director at the Center for Advanced Genetics, an in vitro fertilization program based in Carlsbad, California. He is registered and/or licensed for medicine in California, New York and the United Kingdom.

Table of contents (28 chapter)
1.The Development of PGD
2.Elements of Informed Consent for Preimplantation Genetic Diagnosis
3.Controlled Ovarian Stimulation for Follicular Recruitment and Oocyte Recovery in IVF
4.Biomarker-Based Flow Cytometric Semen Analysis for Male Infertility Diagnostics and Clinical Decision Making in ART
5.Comparison of Methods for Assessment of Sperm DNA Damage (Fragmentation) and Implications for the Assisted Reproductive Technologies
6.Single Gamete Insemination Aiming at the Ideal Conceptus
7.Comprehensive Chromosomal Screening from Polar Body Biopsy to Blastocyst Trophectoderm Sampling: Evidences and Considerations
8.Polar Body Diagnosis (PBD): An Alternative and Supplement to Preimplantation Diagnosis for Single Embryo Transfer
9.Efficiency of Polar Body Biopsy on Aneuploidy Screening by DNA Microarray for Single Euploid Embryo Transfer
10.Prediction of Embryo Viability by Morphokinetic Evaluation to Facilitate Single Transfer
11.Utilisation of Transcriptome-Based Biomarkers for Single Embryo Transfer
12.Array CGH and Partial Genome Sequencing for Rapidly Karyotyping IVF Blastocysts Before Single Transfer
13.Current and Future Preimplantation Genetic Screening (PGS) Technology: From Arrays to Next-Generation Sequencing
14.SNP Array, qPCR, and Next-Generation Sequencing-Based Comprehensive Chromosome Screening
15.Expanding PGD Applications to Nontraditional Genetic and Non-genetic Conditions
16.Should Molecular Cytogenetic Techniques Be Applied to Facilitate Single Embryo Transfer in Egg Donation Cases? Assessment of Frequency and Distribution of Embryo Aneuploidy After Anonymous Donor Oocyte IVF
17.Selecting the Right Embryo in Mitochondrial Disorders
18.Single Embryo Transfer: Significance of the Embryo Transfer Technique
19.The Vitrification Component: An Integral Part of a Successful Single-Embryo Transfer Program
20.A Review of Luteal Support Protocols for Single Embryo Transfers: Fresh and Frozen
21.Cost-Effectiveness of Single Embryo Transfers Relative to Higher Embryo Transfer Policies in Clinical Practice: A Population-Based Analysis
22.The Quebec Experience—One Plus One Equals Two at Once: Presenting Cumulative Pregnancy Rates as the Ideal Outcome in Elective SET Programmes
23.Regulatory Aspects of Embryo Transfer: An Israeli View
24.Single Embryo Transfer: The Québec Experience
25.Regulatory Aspects of Embryo Testing: An American View
26.The Ethical and Legal Analysis of Embryo Preimplantation Testing Policies in Europe
27.Preimplantation Genetic Screening for the Single Embryo: Aims and Responsibilities
28.Crossing the Rubicon: Assisted Reproductive Technologies and Remaining Human