• Part I DNA Synthesis and Genome Engineering 1
1 Competition and the Future of Reading and Writing DNA 3
2 Trackable Multiplex Recombineering (TRMR) and Next-Generation Genome Design Technologies: Modifying Gene Expression in E. coli by Inserting Synthetic DNA Cassettes and Molecular Barcodes 15
3 Site-Directed Genome Modification with Engineered Zinc Finger Proteins 33
4 Rational Efforts to Streamline the Escherichia coli Genome 49
5 Functional Requirements in the Program and the Cell Chassis for Next-Generation Synthetic Biology 81
• Part II Parts and Devices Supporting Control of Protein Expression and Activity 107
6 Constitutive and Regulated Promoters in Yeast: How to Design and Make Use of Promoters in S. cerevisiae 109
7 Splicing and Alternative Splicing Impact on Gene Design 131
8 Design of Ligand-Controlled Genetic Switches Based on RNA Interference 169
9 Small Molecule-Responsive RNA Switches (Bacteria): Important Element of Programming Gene Expression in Response to Environmental Signals in Bacteria 181
10 Programming Gene Expression by Engineering Transcript Stability Control and Processing in Bacteria 189
11 Small Functional Peptides and Their Application in Superfunctionalizing Proteins 217
• Part III Parts and Devices Supporting Spatial Engineering 237
12 Metabolic Channeling Using DNA as a Scaffold 239
13 Synthetic RNA Scaffolds for Spatial Engineering in Cells 261
14 Sequestered: Design and Construction of Synthetic Organelles 279
• Part IV Early Applications of Synthetic Biology: Pathways, Therapies, and Cell-Free Synthesis 307
15 Cell-Free Protein Synthesis: An Emerging Technology for Understanding, Harnessing, and Expanding the Capabilities of Biological Systems 309
16 Applying Advanced DNA Assembly Methods to Generate Pathway Libraries 331
17 Synthetic Biology in Immunotherapy and Stem Cell Therapy Engineering 349
• Part V Societal Ramifications of Synthetic Biology 373
18 Synthetic Biology: From Genetic Engineering 2.0 to Responsible Research and Innovation 375
The inaugural volume of this new reference work in biotechnology is the most comprehensive of its kind on the market, covering everything from DNA synthesis to RNA interference and biosensors. Edited by the renowned scientists Sven Panke of the Swiss Federal Institute of Technology and Christina Smolke from Stanford University.
• Sang Yup Lee is Distinguished Professor at the Department of Chemical and Biomolecular Engineering at the Korea Advanced Institute of Science and Technology (KAIST).
• Jens Nielsen is Professor and Director to Chalmers University of Technology, Sweden. He has received numerous Danish and international awards including the Nature Mentor Award.
• Professor Gregory Stephanopoulos is the W. H. Dow Professor of Chemical Engineering at the Massachusetts Institute of Technology and Director of the MIT Metabolic Engineering Laboratory.