UBIQUITIN-DEPENDENT PROTEIN DEGRADATION (METHODS IN ENZYMOLOGY, VOL. 619)

UBIQUITIN-DEPENDENT PROTEIN DEGRADATION (METHODS IN ENZYMOLOGY, VOL. 619)

Editorial:
ACADEMIC PRESS
Año de edición:
Materia
Biología - Bioquímica
ISBN:
978-0-12-818667-1
Páginas:
378
N. de edición:
1
Idioma:
Inglés
Disponibilidad:
Disponible en 10 días

Descuento:

-5%

Antes:

184,60 €

Despues:

175,37 €

1. Assays for protein retrotranslocation in ERAD
2. Methods for measuring misfolded protein clearance in the budding yeast
3. Methodologies to monitor protein turnover at the inner nuclear membrane
4. Assays for dissecting the in vitro enzymatic activity of yeast Ubc7
5. Methods for genetic analysis of mammalian ER-associated degradation
6. A dual system to manipulate protein levels for DNA replication- and cell cycle-related studies
7. Engineered disulfide crosslinking to measure conformational changes in the 26S proteasome
8. Assays for ubiquitin-like protein ligation and proteasome function in archaea
9. Structural mass spectrometry approaches to study the 20S proteasome
10. Single-molecule methods for measuring ubiquitination and protein stability
11. In vitro analysis of proteasome-associated USP14 activity for substrate degradation and deubiquitylation
12. Methods for studying the regulation of membrane traffic by ubiquitin and the ESCRT pathway
13. Detection of ubiquitinated targets in mammalian and models
14. Western blot analysis of the autophagosomal membrane protein LGG-1/LC3 in
15. Monitoring stress-induced autophagic engulfment and degradation of the 26S proteasome in mammalian cells

• Provides the authority and expertise of leading contributors from an international board of authors
• Presents the latest release in this series on enzymology

Author
Mark Hochstrasser, Eugene Higgins Professor of Molecular Biophysics and Biochemistry and Professor of Molecular, Cellular, and Developmental Biology, Yale University, USA